Reagents: Reaction buffer:5 M IsQHpH 8.0) with 5 mMMng 2. Alkaline phosphatase: 500 pg/mL stock solution in reaction buffer. p-nitmpneummspnatez 1000…

I need to use the dilution equation to fill out the dilution tables that are attached but I do not know what to do.

  • Attachment 1
  • Attachment 2

Reagents:1. Reaction buffer: 0.5 M IflsflQHpH 8.0) with 5 mMMng2. Alkaline phosphatase: 500 pg/mL stock solution in reaction buffer.3. p-nitmpneummspnatez 1000 pM and 10.000 1.1M stock solution in reaction buffer. A. Enzyme solutions (See lab handout Section A-3 for details.) Alkaline phosphatase 0L) Buffer (pL) Total volume (pL) 200020003 100 | 2000 B. Substrate solutions (See lab handout Section A-4 for details.) # [Subsntnrate] p-W Eagle Total vlolume1 10 100 10003 50 100 _ 10004 1 100 10006 100 _ 10007 100 1000